Protein Analyzer

Quantifying or identifying the absence or presence of proteins by directly measuring absorbance or fluorescence provides for fast, convenient results which are critical for biopharmaceutical and bioprocess applications.  Our protein analyzers are used primarily for chromatography and TFF (Tangential  Flow Filtration) applications.

CST’s Protein Analyzers are configured to measure the absorbance of protein concentrations at 280 nm exclusively or at two wavelengths simultaneously which provides users with a more accurate concentration estimate and a larger dynamic range.  Other UV wavelengths available include: 254 nm, 260 nm, 300 nm, 310 nm, 313 nm, 320 nm.  We can also measure at 880 nm for turbidity filtration applications.  A PX2+ can be configured to measure two different wavelengths with two independent path lengths.  By utilizing multiple wavelengths and path lengths, users can monitor protein concentrations from low ppm to percent levels.

Monitoring protein concentrations by measuring their absorbance is an excellent method because it is rapid, simple, and nondestructive to the sample.  The PX2 does not require calibration and can be auto-tuned or zeroed by pressing a button on the back of the unit.  This provides users with a robust solution for UV protein monitoring.

The Protein Analyzer can also be configured to make fluorescence measurements of low concentrations of proteins without the need for dyes or labeling.  This is achieved by utilizing the intrinsic fluorescence of proteins.  This analyzer has been deployed to determine protein concentration, monitor protein folding, protein-protein interactions, protein aggregation and lyophilization.  The analyzer can also be used for genetically encoded autoflourescent proteins (AFP’s) applications.  It has also found utility to detect real-time fouling in downstream biotherapeutic purifications resins.  Superior sensitivity, low level and trace detection, and rapid response summarize the figures of merits of this technology.  CST’s Front Surface Fluorescence Probe coupled with a PX2+ Photometer makes for a robust, highly sensitive, and non-invasive analyzer capable of continuously and accurately measuring protein concentrations.

CST’s Protein Analyzers are designed for continuous, real-time monitoring of protein concentrations.

Other wavelengths available upon request!